## Parameters

## Description

## Equation

## Calculate it!

**Generation Time**

The generation time is the time interval required for the cells to divide.

**Specific growth rate**

Specific growth rate constant is a way of measuring how fast the cells are dividing in a culture.
It is defined on the basis of doubling rate.

**Number of generations**

Growth is by geometric progression: 1, 2, 4, 8, etc. or 2^{0}, 2^{1}, 2^{2}, 2^{3}.........2^{n} (where n = the number of generations).
This is called exponential growth.

**Starting OD calculation**

If we expect for example OD: 2,5 after 10h this equation is calculate the starting OD.
Specific growth rate is required.

**Calculation of suggested inoculation volume**

V_{f} = Media volume [mL]

N = Desired final OD

N_{s} = OD of seed culture

µ = Max growth rate [h^{-1}]

t = Incubation time [h]

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