The generation time is the time interval required for the cells to divide.
Specific growth rate
Specific growth rate constant is a way of measuring how fast the cells are dividing in a culture.
It is defined on the basis of doubling rate.
Number of generations
Growth is by geometric progression: 1, 2, 4, 8, etc. or 20, 21, 22, 23.........2n (where n = the number of generations).
This is called exponential growth.
Starting OD calculation
If we expect for example OD: 2,5 after 10h this equation is calculate the starting OD.
Specific growth rate is required.
Calculation of suggested inoculation volume
Vf = Media volume [mL]
N = Desired final OD
Ns = OD of seed culture
µ = Max growth rate [h-1]
t = Incubation time [h]