The generation time is the time interval required for the cells to divide.

G = ?

Specific growth rate constant is a way of measuring how fast the cells are dividing in a culture. It is defined on the basis of doubling rate.

µ = ?

Growth is by geometric progression: 1, 2, 4, 8, etc. or 2⁰, 2¹, 2², 2³.........2ⁿ (where n = the number of generations). This is called exponential growth.

n = ?

If we expect for example OD: 2,5 after 10h this equation is calculate the starting OD. Specific growth rate is required.

N₀ = ?

V_f = Media volume [mL]
N = Desired final OD
N_s = OD of seed culture
µ = Max growth rate [h^-1]
t = Incubation time [h]