Methods to Prepare Buffer Solutions

100 mM phosphoric acid (sodium) buffer solution (pH=2.1)
Sodium dihydrogen phosphate dihydrate (M.W.=156.01)..50 mmol (7.8 g)
Phosphoric acid (85 %, 14.7 mol/L)…………………….50 mmol (3.4 mL)
Add water to make up to 1 L.

10 mM phosphoric acid (sodium) buffer solution (pH=2.6)
Sodium dihydrogen phosphate dihydrate (M.W.=156.01)..5 mmol (0.78 g)
Phosphoric acid (85 %, 14.7 mol/L)…………………….5 mmol (0.34 mL)
Add water to make up to 1 L.
(Alternatively, dilute 100 mM phosphoric acid (sodium) buffer solution (pH=2.1) ten times.)

50 mM phosphoric acid (sodium) buffer solution (pH=2.8)
Sodium dihydrogen phosphate dihydrate (M.W.=156.01)..40 mmol (6.24 g)
Phosphoric acid (85 %, 14.7 mol/L)…………………….10 mmol (0.68 mL)
Add water to make up to 1 L.

100 mM phosphoric acid (sodium) buffer solution (pH=6.8)
Sodium dihydrogen phosphate dihydrate (M.W.=156.01)..50 mmol (7.8 g)
Sodium dihydrogen phosphate 12-hydrate (M.W.=358.14)..50 mmol (17.9 g)
Add water to make up to 1 L.

10 mM phosphoric acid (sodium) buffer solution (pH=6.9)
Sodium dihydrogen phosphate dihydrate (M.W.=156.01)..5 mmol (0.78 g)
Sodium dihydrogen phosphate 12-hydrate (M.W.=358.14)..5 mmol (1.79 g)
Add water to make up to 1 L.
(Alternatively, dilute 100 mM phosphoric acid (sodium) buffer solution (pH=6.8) ten times.)

20 mM citric acid (sodium) buffer solution (pH=3.1)
Citrate dihydrate (M.W.=210.14)……………16.7 mmol (3.51 g)
Sodium citrate dihydrate (M.W.=294.10)..3.3 mmol (0.97 g)
Add water to make up to 1 L.

20 mM citric acid (sodium) buffer solution (pH=4.6)
Citrate dihydrate (M.W.=210.14)……………10 mmol (2.1 g)
Sodium citrate dihydrate (M.W.=294.10)..10 mmol (2.94 g)
Add water to make up to 1 L.

10 mM tartaric acid (sodium) buffer solution (pH=2.9)
Tartaric acid (M.W.=150.09)……………………..7.5 mmol (1.13 g)
Sodium tartrate dihydrate (M.W.=230.08)……..2.5 mmol (0.58 g)
Add water to make up to 1 L.

10 mM tartaric acid (sodium) buffer solution (pH=4.2)
Tartaric acid (M.W.=150.09)……………………..2.5 mmol (0.375 g)
Sodium tart rate dihydrate (M.W.=230.08)……..7.5 mmol (1.726 g)
Add water to make up to 1 L.

20mM (acetic acid) ethanolamine buffer solution (pH=9.6)
Monoethanolamine (M.W.=61.87, d=1.017)…20 mmol (1.22 mL)
Acetic acid (glacial acetic acid, 17.4 mol/L)………………10 mmol (0.575 mL)
Add water to make up to 1 L.

100 mM acetic acid (sodium) buffer solution (pH=4.7)
Acetic acid (glacial acetic acid) (99.5 %, 17.4 mol/L)………………50 mmol (2.87 mL)
Sodium acetate trihydrate (M.W.=136.08)……..50 mmol (6.80 g)
Add water to make up to 1 L.

100 mM boric acid (potassium) buffer solution (pH=9.1)
Boric acid (M.W.=61.83)……………100 mmol (6.18 g)
Potassium hydroxide (M.W.=56.11)……………50 mmol (2.81 g)
Add water to make up to 1 L.

100 mM boric acid (sodium) buffer solution (pH=9.1)
Boric acid (M.W.=61.83)……………100 mmol (6.18 g)
Sodium hydroxide (M.W.=40.00)……………50 mmol (2.00 g)
Add water to make up to 1 L.

 

phosphate buffer preparation (pH5.8-8)  PDF
http://www.shimadzu.com/an/hplc/support/lib/lctalk/38/38lab.html

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